Jannes Höpke's Master thesis was awarded with the Prize for the Best Plant Science Master Thesis, which was carried out at Carl von Ossietzky-Universität Oldenburg in the year 2019.
The intricate species boundary between Veronica spicata and V. orchidea (Plantaginaceae)
For the fist time a systematical comparison regarding missing data in combination with missing allele-dosis-information for population genetical methods like PCA/PCoA and STRUCTURE was conducted. Moreover, genetical material from the type localities of V. barrelieri subsp. crassifolia und V. tzesnae was analysed for the first time in this study.
The aim was to analyse the potentially hybridising species Veronica spicata and V. orchidea (Plantaginaceae) regarding their species boundary. Therefore, both species and morphologically similar species were analysed using Genotyping-by-Sequencing (GBS). The same question was already analysed by Bardy et al. (2011) using AFLP data but we were not able to completely confirm their results. To get a better understanding of how mixed ploidy-levels, the influence of missing data, and missing allele-dosis-information influence population genetical analyses, simulations and analyses of GBS data were conducted to reveal the reasons for the observed differences. Hereby a bug in the programme ipyrad was found out, the importance of paralogs was analysed and the difference between the coding of AFLP and diploidised SNP-Data was worked out
Jannes Höpke conducted this work in the Institute for Biology and Environmental Sciences in the working group Biodiversity and Evolution of Plants of Prof. Dr. Dirk Albach
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