Within the Open Science project a multidisciplinary study analyzed Ballota acetabulosa (L.) Benth. at the Ghirardi Botanic Garden (Toscolano Maderno, BS, Italy). Micromorphological and histochemical investigations were performed on the secreting structures of the vegetative and reproductive organs under light, fuorescence and electronic microscopy. Concurrently spontaneously emitted volatiles from leaves and flowers were examined. Four trichome morphotypes were identified: peltate and short-stalked, mediumstalked and long-stalked capitate trichomes, each with a specific distribution pattern. The histochemical analysis was confirmed using ultrastructural observations, with the peltates and long-stalked capitates as the main sites responsible for terpene production. The head-space characterization revealed that sesquiterpene hydrocarbons dominated both in leaves and flowers, with c-muurolene, b-caryophyllene and (E)-nerolidol as the most abundant compounds. Moreover, a comparison with literature data concerning the ecological roles of the main compounds suggested their dominant roles in defence, both at the leaf and flower level.
Read whole paper "paper "Micromorphological and phytochemical survey of Ballota acetabulosa (L.) Benth" in our scientific journal Plant Biology 23 (2021) 643–65. DOI: https://doi.org/10.1111/plb.13254